Dark variants of luminous bacteria whole cell bioluminescent optical fiber sensor to genotoxicants.
- Author:
Yaliang SUN
1
;
Tiebo ZHOU
;
Jianli GUO
;
Yiyong LI
Author Information
1. Department of Chemistry, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
Biosensing Techniques;
Fiber Optic Technology;
Genetic Variation;
Luminescent Measurements;
Luminescent Proteins;
genetics;
Mitomycin;
pharmacology;
toxicity;
Optical Fibers;
Photobacterium;
genetics;
Transcription, Genetic;
drug effects
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(5):507-509
- CountryChina
- Language:English
-
Abstract:
A stable dark variant separated from photobacterium phosphoreum (A2) was fixed in agar-gel membrane and immobilized onto an exposed end of a fiber-optic linked with bioluminometer. The variant could emit a luminescent signal in the presence of genotoxic agents, such as Mitomycin C (MC). The performance of this whole-cell optical fiber sensor system was examined as a function of several parameters, including gel probe thickness, bacterial cell density, and diameter of the fiber-optic core and working temperature. An optimal response to a model genotoxicant, Mitomycin C, was achieved with agar-bacterial gel membrane: the thickness of gel membrane was about 5 mm; the cell density of bacteria in gel membrane was about 2.0 x 10(7)/ml; the diameter of fiber-optic core was 5.0 mm; the working temperature was 25 degrees C. Under these optimized conditions, the response time was less than 10 h to Mitomycin C, with a lower detection threshold of 0.1 mg/L.
