Transcription and translation of Dickkopf-1 in endometrium of pregnant mice during the peri-implantation period.
- Author:
Hanwang ZHANG
1
;
Qiaohong LAI
Author Information
1. Department of Obstetrics & Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Embryo Implantation;
genetics;
Endometrium;
metabolism;
Female;
Intercellular Signaling Peptides and Proteins;
biosynthesis;
genetics;
Male;
Mice;
Pregnancy;
Protein Biosynthesis;
RNA, Messenger;
genetics;
Transcription, Genetic
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(6):625-638
- CountryChina
- Language:English
-
Abstract:
To study the expression of Dickkopf-1 (DKK-1) in endometrium of pregnant mice during the peri-implantation period and the role of DKK-1 during the embryo implantation in mice. Immunohistochemical technique was employed to determine the location of DKK-1 protein in endometrium, and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was utilized to determine the levels of DKK-1 mRNA. Our results showed that the expressions of DKK1 mRNA and protein were higher in experimental groups than in control group (P<0.01) and it increased significantly on day 3 and reached its peak on day 4, and then decreased gradually on day 5-7. The levels of DKK-1 mRNA and protein on day 4 was significantly higher than those of other groups (P<0.01). It is concluded that DKK-1 probably plays an important role in signal transudation of embryo implantation and its high expression indicates the opening of implantation window.
