Expression of Cyclooxygenase-2 mRNA and Identification of Its Splice Variant in Human Myometrium Obtained from Women in Labor
- Author:
Yinping HUANG
1
;
Duyun YE
;
Ping WU
;
Yanjun HUANG
;
Li ZHANG
;
Xiaoyan ZHOU
;
Yunfeng HUANG
;
Ping YUAN
;
Daijuan ZHANG
;
Jingyuan WAN
Author Information
1. First Affiliated Hospital of Wenzhou Medical College
- Keywords:
cyclooxygenase-2 gene;
splice variant;
gene cloning;
sequencing;
myometrium
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(1):5-7
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing.
