Effect of Angiotensin Ⅱ on Cord Blood CD34+ Cells Expansion in vitro
- Author:
Cheng PENG
1
;
Ping ZOU
;
Yanping MA
;
Zongbo HU
Author Information
1. Union Hospital Tongji Medical College Huazhong University of Science and Technology
- Keywords:
angiotensin Ⅱ;
cord blood hematopoietic stem/progenitor cell;
ex vivo expansion
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(1):26-28
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the influence of angiotensin Ⅱ on hematopoietic system, CD34+cells in cord blood were purified, and the effects of angiotensin Ⅱ in combination with various cytokines on their growth and differentiation were studied by cell culture in vitro. It was found that angiotensin Ⅱ in suspending medium could stimulate both BFU-E and CFU-GM expansion. The number of BFU-E and CFU-GM was increased with the increases of angiotensin Ⅱ concentrations during a certain range. In addition, the expansion fold of CFU-GM was increased from 2.3±0.8 times to 7.8±2.3 times when angiotensin Ⅱ was added in the presence of SCF+G-CSF+GM-CSF+IL-3 cytokines mixture. Similarly, the expansion fold of BFU-E was increased from 3.1 ±1.8 times to 9.2±2.3 times with angiotensin Ⅱ in the presence of SCF+EPO+TPO+IL-3. In the semi-solid medium, angiotensin Ⅱ could stimulate CFU-GM expansion but had no effect on the growth of BFU-E. In conclusion, angiotensin Ⅱ had some stimulating effects on cord blood hematopoietic progenitors expansion in vitro in the presence of other cytokines.
