Effects of 2-APB on Store-operated Ca2+ Channel Currents of Hepatocytes after Hepatic Ischemia/Reperfusion Injury in Rats
- Author:
Changzhou HUANG
1
;
Zongming ZHANG
;
Fazu QIU
Author Information
1. Tongji Hospital Tongji Medical College Huazhong University of Science and Technology
- Keywords:
hepatic ischemia/reperfusion injuries;
hepatocytes;
store-operated calcium current;
store-operated calcium channel;
calcium channel blockers
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(1):39-41
- CountryChina
- Language:Chinese
-
Abstract:
The effects of hepatic ischemia/reperfusion (I/R) injuries on hepatocellular viability and store-operated calcium current (Isoc) in isolated rat hepatocytes and the effects of 2-APB on storeoperated calcium current (Isoc) in isolated rat hepatocytes after hepaticischemia/reperfusion injuries were studied. Hepatic ischemia and reperfusion injury model was established and whole cell patch-clamp techniques were used to investigate the effects of 2-APB on Isoc. The results showed that ischemia/reperfusion injuries could significantly reduce hepatocellular viability and further increase Isoc in hepatocytes and 2-APB (20, 40, 60, 80, 100 μmol/L) produced a concentration-dependent decrease of Isoc with IC50 value of 64.63±10.56 μmol/L (n= 8). It was concluded that ischemia/reperfusion injuries could reduce hepatocellular viability, probably through increased Isoc in hepatocytes and 2-APB had a protective effect on ischemia/reperfusion-induced liver injury, probably though inhibiting Isoc.
