Effects of Oxidized Low Density Lipoprotein on the Expression and Function of ABCA1 in Macrophages
- Author:
Yinghong LI
1
;
Hao BI
;
Fan WU
;
Yiqiang ZONG
;
WANGYan
;
Shen QU
Author Information
1. Tongji Medical College Huazhong University of Science and Technology
- Keywords:
ATP binding cassette transporter A1;
cholesterol efflux;
low density lipoprotein;
gene expression regulation
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(2):113-116
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, we examined the regulation of the expression and function of AB CA1 by modified LDL (ox-LDL) in vitro. After incubation with apoA-Ⅰ for 24 h, RAW264.7 cells effluxed 37.65 % cholesterol loaded by acetyl LDL (ac-LDL), and 9.78 % cholesterol in ox-LDL group. The level of ABCA1 Mrna increased about three times either when cells were incubated with 100 μg/Ml ac-LDL or with 100μg/Ml ox-LDL. However, the level of ABCA1 protein rose by 1.57 times in ac-LDL group and 1.26 times in ox-LDL group. These results demonstrated that ox-LDL had different effect on the expression and function of ABCA1, ox-LDL might decrease the cholesterol efflux mediated by ABCA1 through other unknown mechanisms.
