Inhibition of cell growth by rapamycin through targeting mammalian target of rapamycin signaling pathway in nasopharyngeal carcinoma
10.3760/cma.j.issn.1673-0860.2010.09.013
- VernacularTitle:雷帕霉素靶向阻断作用对鼻咽癌细胞生长的实验研究
- Author:
Yan-Li LI
1
;
Xin ZHANG
;
Yong LIU
;
Shi-Sheng LI
;
Li XIE
;
Ning ZHANG
;
Xue-Bing LIU
;
Yong-Quan TIAN
Author Information
1. 北京市仁和医院
- Keywords:
Nasopharyngeal neoplasms;
Sirolimus;
Cell line,tumor
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(9):765-768
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of rapamycin on cell growth and cell cycle in CNE-1 and CNE-2 cells. Methods Growth inhibition effect of rapamycin on CNE-1 and CNE-2 cells were assessed by cell counting kit-8 (CCK-8) assay. Morphological alterations of the cells were observed by microscope. Cell cycle and cell apoptosis were analyzed by FCM. The expression of mammalian target of rapamycin (mTOR)was analyzed by reverse transcription-polymerase chain reaction(RT-PCR). Results The growth of CNE-1and CNE-2 cells was inhibited significantly by rapamycin dose-dependently. FCM showed that CNE-1 and CNE-2 cells at 48 hours after rapamycin ( 150 nmoL/L) treatment were arrested in the G0/G1 phase of cell cycle. However rapamycin treatment did not significantly induce apoptosis of CNE-1 and CNE-2 cells (P ＞0. 05). RT-PCR showed that rapamycin significantly inhibited mRNA expression of mTOR in CNE-2 cells (t = 10. 625 ,P ＜ 0. 01 ). Conclusions Rapamycin inhibits the growth of CNE-1 and CNE-2 cells by inhibiting the progression of cell cycle, which could be achieved through decreaseing the expression of mTOR.
