Effect of brain-derived neurotrophic factor gene transfected bone-marrow mesenchymal stem cells on damaged cochlear spiral ganglion cells of guinea pigs
10.3760/cma.j.issn.1673-0860.2010.12.013
- VernacularTitle:脑源性神经营养因子基因转染骨髓间充质干细胞对豚鼠受损耳蜗螺旋神经节细胞的影响
- Author:
Qian-Xu LIU
1
;
Guan-Gui CHEN
;
Xiang-Bo HE
;
Ding-Hua XIE
;
Zhi-Qiang TAN
Author Information
1. 珠海市人民医院
- Keywords:
Brain-derived neurotrophic factor;
Mesenchymal stem cells;
Spiral ganglion;
Mesenchymal stem cell transplantation;
Aminoglycosides
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(12):1029-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective role of brain-derived neurotrophic factor (BDNF) gene transfected bone-marrow mesenchymal stem cells (BMSC) on cochlear spiral ganglion cells (SGC) impaired by aminoglycoside antibiotics(AmAn). Methods The differentiation of BMSC transfected by BDNF gene (BDNF-BMSC) were detected with immunohistochemical examination of Nestin, neuronspecific enolase (NSE), and glial fibrillary acid protein (GFAP)antibody in vitro. BDNF gene transfected BMSC were transplanted into the cochleae of guinea pigs deafened by amikacin, while the control groups were designed in which artificial perilymphatic fluid(APF), BMSC or BDNF gene was injected into cochleae alone. The cochleae were obtained on the week 1,2 and 4 after injection, respectively, paraffin-embedded,and cut in a paramodiolar plane subsequently. The histopathological changes of cochleae were observed, the density of SGC was calculated by staining with HE, and the corresponding optical density (COD) was calculated with immunohistochemical staining using NSE antibody. And the protective role of various groups on the cochlear SGC were compared. Results The positive staining rate of BDNF gene transfected BMSC with Nestin, NSE and GFAP antibody were all higher than that of BMSC in vitro (P< 0.01). After transplantation into cochleae, the differences of SGC density and COD among various groups were all significant on the same time points(P < 0.05). The SGC density and COD of the BDNF gene transfected BMSC group were the highest. The SGC density and COD of various groups on week 4 were all obviously decreased than those on week 1 and 2 (P < 0.05). Conclusion AmAn-induced SGC damage could be depressed by BMSC, BDNF gene or BDNF gene transfected BMSC transplantion into cochleae, while BDNF gene transfected BMSC showed the best protective role.
