Expression of metabotropic glutamate receptor 4 in cardiomyocytes differentiated from mouse embryonic stem cells in vitro.
- Author:
Bei ZHENG
1
;
Li-min ZHOU
;
Qing-wei ZHAO
;
Xing-guo ZHANG
;
Dan-yan ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; physiology; Cell Line; Embryonic Stem Cells; cytology; metabolism; Mice; Mice, Inbred ICR; Myocytes, Cardiac; cytology; metabolism; RNA, Messenger; genetics; Receptors, Metabotropic Glutamate; genetics; metabolism
- From: Journal of Zhejiang University. Medical sciences 2012;41(4):366-372
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of metabotropic glutamate receptor 4 (mGluR4) in cardiomyocytes differentiated from mouse embryonic stem cells (ES cells).
METHODSES cells were differentiated into cardiomyocytes with hanging-drop cultures. Retinoic acid (RA) and dimethyl sulfoxide (DMSO) were used as positive and negative controls, respectively. The co-expression of cardiac sarcomeric protein (α-actinin or troponin-T) and mGluR4 were verified by immunocytochemistry and flow cytometry analysis. The mRNA and protein expressions of mGluR4 were verified by RT-PCR and Western blot analysis, respectively. Meanwhile, the expression of mGluR4 in prenatal mouse heart was also examined.
RESULTSmGluR4 was expressed in both mouse ES cells and ES cell-derived cardiomyocytes. The level of mGluR4 protein expression decreased during the maturation of the cardiomyocytes. The co-expression rate of mGluR4 and Troponin T in the beating embryoid bodies (EBs) was only (3.00 ±1.00)%. On the other hand, mGluR4 gene and protein expressions showed remarkable down-regulation in the development of mouse fetal heart, which was not detected in mouse adult heart.
CONCLUSIONThe expression of mGluR4 is down-regulated in the cardiomyocyte differentiation of ES cells. The trend of expression is consistent with that in the prenatal mouse heart development.
