A method for simultaneous assay of propulsion and absorption in small intestine.
- Author:
Jian-xiang LIU
1
;
Xin-ye ZHANG
;
Dong-mei PAN
;
Qin-li ZHANG
;
Xiang-rong LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biological Assay; methods; Intestinal Absorption; Intestine, Small; metabolism; physiology; Male; Mice; Mice, Inbred ICR; Peristalsis; Phenolsulfonphthalein; pharmacokinetics; Xylose; pharmacokinetics
- From: Journal of Zhejiang University. Medical sciences 2012;41(4):430-449
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for simultaneous assay of propulsion and absorption in small intestine.
METHODSThe mice were administrated through gastric tube with mixed reagents containing 0.12% phenol red, D-xylose (1.25%, 2.5% and 5%) and 15% gelatin. The influence of phenol red on D-xylose absorption and the influence of D-xylose on small intestine propulsion rate were investigated by measuring serum concentration of D-xylose with phloroglucinol method.
RESULTSAt 10 min, no significant difference was found between 5% D-xylose mixed reagent group and 5% D-xylose control. At 15 min, small intestine propulsion rate in 5% D-xylose mixed reagent group, but not in 2.5% and 1.25% D-xylose mixed reagent groups, was significantly higher than in phenol red control (P<0.05).
CONCLUSIONGastric administration of mixed reagent containing 0.12% phenol red, 5% D-xylose and 15% gelatin can simultaneously assay propulsion and absorption of small intestine in mice.
