Screening and identification of high-yield poly(β-malic acid) bacterial strain.
- Author:
Ling-fen XU
1
;
Zhong-hua CHEN
;
Jing ZHAO
;
Hong RUAN
;
Sen-quan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Fermentation; Malates; metabolism; Mitosporic Fungi; isolation & purification; metabolism; Polymers; metabolism
- From: Journal of Zhejiang University. Medical sciences 2012;41(4):434-440
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and identify the high-yield poly-malic acid (PMLA) bacterial strains from the nature.
METHODSSamples were collected and cultured. The high-yield PMLA bacterial strains were screened through morphological observation, qualitative PMLA tests by HPLC and ITS sequence analysis on the isolated bacterial strains.
RESULTSA high-yield PMLA strain II 04 was isolated, the yield of PMLA of the strain reached to 26.23g/L in the rotary shaker at 25 degree for 7d. From morphological observation and ITS sequences analysis, the strain belonged to Aureobasidium pullulans, and named as Aureobasidium pullulans ZUCC-41.
CONCLUSIONA high-yield bacterial strain has been isolated from the nature and identified to be Aureobasidium pullulans.
