Antitumor activity of histone deacetylase inhibitor suberic bishydroxamate on acute myeloid leukemia cell lines.
- Author:
Yan-hua XU
1
;
Chun-mei YANG
;
Wen-bin QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Apoptosis Regulatory Proteins; metabolism; Caspases; metabolism; Cell Line, Tumor; Histone Deacetylase Inhibitors; pharmacology; Humans; Hydroxamic Acids; pharmacology; Leukemia, Myeloid, Acute; metabolism; pathology
- From: Journal of Zhejiang University. Medical sciences 2012;41(5):491-497
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of histone deacetylase inhibitor suberic bishydroxamate (SBHA) on human acute myeloid leukemia (AML) cell lines.
METHODSAML U937, KG-1 and Kasumi-1 cells were treated with SBHA. Cell growth was measured by MTT assay. Apoptosis was determined using flow cytometry. Activation of Caspase pathway and expression of apoptosis regulator proteins were detected by Western blot.
RESULTSSBHA significantly induced growth arrest and apoptosis in U937, KG-1 and Kasumi-1 cells. Enhanced apoptosis was observed in SHBA group evidenced by strong activation of Caspase-9, Caspase-8 and Caspase-3. SHBA treatment resulted in down-regulation of anti-apoptotic protein Bcl-2 and Bcl-xl expression; down-regulated expression of antiapoptotic proteins survivin, XIAP and cIAP was also detected after SBHA treatment.
CONCLUSIONSBHA can effectively kill AML cells by inhibiting cell growth and inducing apoptosis, which is associated with the activation of Caspase pathway and regulation of apoptotic related proteins.
