Effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats.
- Author:
Hui LI
1
;
Ke RAN
;
Zheng-guo TANG
;
Shuang-feng LI
;
Ye-tian CHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Hydrogen Sulfide; pharmacology; Ischemic Preconditioning, Myocardial; Male; Myocardial Reperfusion Injury; metabolism; pathology; prevention & control; Myocardium; metabolism; pathology; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2012;41(5):559-563
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats.
METHODSSprague-Dawley male rats were divided into 4 groups with 10 in each group: in S group rats received sham operation; in IR group rats were given with NS (1.0 ml/kg iv) 24 h before ischemia; in H group rats were treated with NaHS (0.05 mg/kg iv) 24 h before ischemia; and in D group, NaHS-treated rats received 5-hydroxydecanoate (5-HD) 15 min before ischemia. Rats in IR group,H group and D group were subjected to ischemia by occlusion of coronary artery for 30 min followed by 2 h of reperfusion. At the end of the reperfusion,myocardial infarct size was measured. SAM-s was measured by Western blotting. Plasma SOD activity and MDA were determined at the end of reperfusion.
RESULTSThe infarct size was significantly lesser in H group (25.40 % ± 3.54%) than that in IR group (38.27% ±5.64%,P<0.05). The SAM-s protein expression in myocardium was significantly lower in H group than that in IR group. The plasma MDA content was significantly lower and SOD activity was higher in H group than those in IR group,but there was no difference between IR group and D group.
CONCLUSIONThe hydrogen sulfide preconditioning attenuates myocardial IR injury possibly through down-regulating SAM-s expression,reducing the production of oxygen free radicals and enhancing anti-oxidize effect in rats.
