Corbrin shugan capsule for treatment of alcoholic hepatic fibrosis in rats.
- Author:
Rong HU
1
;
Xu-chun FU
;
Li-mei SHEN
;
Hai-bo BAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Drugs, Chinese Herbal; therapeutic use; Hydroxyproline; metabolism; Laminin; blood; Liver; metabolism; pathology; Liver Cirrhosis, Alcoholic; drug therapy; metabolism; pathology; Male; Procollagen; blood; Rats; Tissue Inhibitor of Metalloproteinase-1; blood
- From: Journal of Zhejiang University. Medical sciences 2012;41(5):564-568
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the therapeutic effect of Corbrin shugan capsule for treatment of alcoholic hepatic fibrosis in rats.
METHODSThe rat model of alcoholic hepatic fibrosis was induced by intragastric administration of alcohol repeatedly. The serum procollagen III (PC III), laminin (LN) and tissue inhibitors of metalloproteinase-1 (TIMP-1) levels were measured with ELISA, and the content of hydroxyproline (Hyp) in liver tissue were determined with colorimetric method. Collagen deposition in liver tissue was observed with Masson's staining, and the fibrosis area was measured with digital medical image analysis system (Motic Med 6.0).
RESULTSCompared with the model control group, the serum TIMP-1 and LN levels and hepatic fibrosis area in liver tissue significantly decreased in Corbrin shugan capsule groups with doses of 0.09,0.27 and 0.45 g*kg(-1), and the serum PC III and the Hyp contents in liver tissue also decreased of Corbrin shugan capsule groups with doses of 0.27 and 0.45g*kg(-1).
CONCLUSIONCorbrin shugan capsule can decrease serum PC III, TIMP-1 and LN levels and Hyp levels in liver tissue and hepatic fibrosis area in rats, indicating it may have therapeutic effect on alcoholic hepatic fibrosis.
