Simultaneous determination of madecassoside, asiaticoside and their aglycones in Centella asiatica (L.) urban extracts by RP-HPLC.
- Author:
Qian SHEN
1
,
2
;
Lu-shan YU
1
;
Hui ZHOU
3
;
Su ZENG
1
;
Author Information
- Publication Type:Journal Article
- MeSH: Centella; chemistry; Chromatography, High Pressure Liquid; methods; Pentacyclic Triterpenes; analysis; Plant Extracts; analysis; Reproducibility of Results; Triterpenes; analysis
- From: Journal of Zhejiang University. Medical sciences 2014;43(2):135-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for simultaneous determination of four major constituents (madecassoside, asiaticoside, madecassic acid and asiatic acid) in Centella asiatica (L.) urban extracts.
METHODSThe analysis was performed on an Agilent 1100 HPLC system with a ZORBAX Eclipse XDB-C8 column (4.6 mm×150 mm, 5μm). The four major constituents were separated with gradient mobile phase that consists of 1mmol/L potassium dihydrogen phosphate and acetonitrile at the detection wavelength of 205 nm.
RESULTSThe four major constituents all had good linear response in the determination ranges (R(2)≥0.9998). The average recoveries (n=9) were 97.4%, 93.7%, 97.5% and 99.8% with RSDs of 3.4%, 1.4%, 4.7% and 4.4%, respectively.
CONCLUSIONThe developed method is sensitive and has good reproducibility, which can be used as a reference for quality control of Centella asiatica (L.) urban extracts.
