Quantitative determination of voglibose contents in its tablets with high-performance liquid chromatography-mass spectrometry.
- Author:
Xiang-qun ZHANG
1
;
Ling-hua WU
1
;
Fang-jun CHEN
1
;
Yi ZHANG
1
;
Lu CHEN
1
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Inositol; analogs & derivatives; analysis; Mass Spectrometry; methods; Tablets
- From: Journal of Zhejiang University. Medical sciences 2014;43(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for determination of voglibose contents in its tablets by high-performance liquid chromatography-mass spectrometry (HPLC-MS).
METHODSThe measurements were carried out on an Agilent ZORBAX Eclipse Plus C18 column (2.1×150mm 3.2μm) with a temperature of 40 degrees Celsius. A mixture of methanol and water (2:3,v/v) was used as a mobile phase at a flow rate of 0.25 ml/min. Voglibose was detected in an electrospray ionization (ESI) mode with MRM.
RESULTSThe calibration curves of voglibose showed good linearity in a range of 1.5804-2.6340 μg/ml (r=0.9990). The average recovery was 100.2% with RSD of 1.37% (n=6) for m/z 268.2/74.2.Linearity was obtained with r=0.9976 and the average recovery was 99.3% with RSD of 1.78% (n=6) for m/z 268.2/92.2.
CONCLUSIONHPLC-MS method is accurate,reproducible and can be used for quality control of voglibose tablets.
