Purification of polyphenols from Sabina vulgaris antoine and its antioxidant properties.

Long LI; Gui-da Xuan; Ping Chen

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Purification of polyphenols from Sabina vulgaris antoine and its antioxidant properties.

Author: Long LI ^{1
,

2} ; Gui-da XUAN ³ ; Ping CHEN ⁴
Author Information

1. 1. School of Medicine, Zhejiang University City College, Hangzhou 310015,China
2. 2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310018, China
3. School of Medicine, Zhejiang University City College, Hangzhou 310015,China.
4. College of Science, Zhejiang University, Hangzhou 310018, China.
Publication Type:Journal Article
MeSH: Antioxidants; pharmacology; Cupressaceae; chemistry; Polyphenols; isolation & purification
From: Journal of Zhejiang University. Medical sciences 2014;43(2):175-179
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties.
METHODSPolyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700, and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry. The antioxidant properties of polyphenols were evaluated by total antioxidant capacity (T-AOC) and its activities of scavenging DPPH (1,1 diphenyl-2-picry-hydrazyl) radicals, superoxide anion (O2·-), hydroxyl free radicals (OH·) and ferric reducing antioxidant power (FRAP).
RESULTSAfter purification, the purity of polyphenols increased from 0.053% to 0.995%.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml. Its scavenging capacity for superoxide anion (O2·-) and hydroxyl free radicals (OH·) was 151.83 U/ml and 204.59 U/ml. The total antioxidant capacity was 72.68 U/ml.
CONCLUSIONPolyphenols from Sabina vulgaris Antoine have high antioxidant properties, suggesting that it worth further study of its pharmacological effects.