Identification of proteins that interact with murine cytomegalovirus early protein M112-113 in brain.
- Author:
Hui WANG
1
;
Xing-Lou LIU
;
Sai-Nan SHU
;
Yong-Jian HUANG
;
Feng FANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; Cell Line; Humans; Immunoprecipitation; Mice; Muromegalovirus; metabolism; Plasmids; Protein Binding; Two-Hybrid System Techniques; Viral Proteins; metabolism
- From: Chinese Medical Journal 2011;124(21):3532-3536
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDMurine cytomegalovirus (MCMV) early protein M112-113 is involved in viral DNA replication and believed to play a crucial role in the viral pathogenesis. To investigate the biological function of M112-113 protein in the pathogenesis of the brain disorders caused by cytomegalovirus (CMV), a screening for proteins interacting with M112-113 was performed by a yeast two-hybrid system.
METHODSBait plasmid pGBKT7-M112-113 was constructed and transformed into AH109 yeast. After confirmation of the expression of MCMV M112-113 in yeast, the bait yeast was mated with a prey yeast containing mouse brain cDNA library plasmid to screen the proteins interacting with M112-113. Interactions between M112-113 and the obtained proteins were verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion.
RESULTSTwo proteins interacting with M112-113 were identified, including metastasis-associated 1 (MTA1) and zinc finger, CCHC domain containing 18 (ZCCHC18). M112-113 protein could interact with MTA1 or ZCCHC18 in yeast and mammalian cells.
CONCLUSIONThe interactions of M112-113 with MTA1 or ZCCHC18 may be related to the pathogenesis of MCMV-associated disease in central nervous system.
