Impact of trichostatin A on gastric carcinoma cell line SGC-7901.
- Author:
Yun-long LI
1
;
Xiao-ming ZOU
;
Bao-liang GUO
;
Xiao-lin LI
;
Chao-qi YAN
;
Li-guang YOU
;
Song-bin FU
Author Information
- Publication Type:Journal Article
- MeSH: Acetylation; drug effects; Apoptosis; drug effects; Cell Line, Tumor; Histones; metabolism; Humans; Hydroxamic Acids; pharmacology; Stomach Neoplasms
- From: Chinese Journal of Gastrointestinal Surgery 2007;10(4):376-379
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of trichostatin A(TSA) on SGC- 7901 cells.
METHODSCytotoxicity and cell viability of gastric cancer cell line SGC- 7901 were assayed by MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope. Cell cycle and apoptosis rate were analyzed by flow cytometry. Histone H3 acetylation was detected by Western blot.
RESULTSTSA showed apparently cytotoxicity in SGC- 7901 cells. The growth curve showed the growth ratio decreased with the increase of TSA concentration. Apoptosis rate were significantly different between TSA treated group(75 ng/ml for 72 h)and control group (P < 0.05). Morphologic changes of apoptosis including nuclear chromatin condensation and fluorescence strength were observed with fluorescence microscope.TSA treatment (75 ng/ml for 72 h) sensitively induced apoptosis in the cell,which was demonstrated by the migration of many cells to the sub- G1 phase,the reduction of G1- phase cells and the increment of apoptosis rate (29.54%) in flow cytometric analysis. The expression of acetylated histone H3 was increased in TSA group(75 ng/ml) for 48 h compared with control group by Western blot.
CONCLUSIONSTSA can induce SGC- 7901 cell apoptosis. The expression of acetylated histone H3 may contribute to the apoptosis.
