Nuclear expression of S100A4 is associated with lymph node metastasis in gastric carcinoma.
- Author:
Xi-yao ZHONG
1
;
Lian-hai ZHANG
;
Shu-qin JIA
;
Tao SHI
;
Hong DU
;
Ying HU
;
Gui-guo ZHANG
;
Ai-ping LU
;
Ji-you LI
;
Jia-fu JI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Nucleus; metabolism; Humans; Lymphatic Metastasis; Neoplasm Staging; S100 Calcium-Binding Protein A4; S100 Proteins; metabolism; Stomach Neoplasms; metabolism; pathology
- From: Chinese Journal of Gastrointestinal Surgery 2007;10(5):454-457
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the intracellular localization of S100A4 in gastric carcinoma cells and the relationship between S100A4 expression status and lymph node metastasis of gastric carcinoma.
METHODSWestern blotting analysis was performed to locate the expression of S100A4 protein in sub-fraction components of frozen tissues. S100A4 protein expression was also determined by immunohistochemical method in 131 samples of gastric cancer and 20 samples of matched metastatic lymph nodes.
RESULTSThirty-two of 131 (24.4%) gastric carcinoma showed positive S100A4 nuclear expression and 50/131 (38.2%) carcinoma showed positive cytoplasmic expression. In 32 samples with positive S100A4 nuclear expression, 30 (93.8%) carcinomas had positive lymph node metastases. S100A4 nuclear expression level was higher in gastric carcinoma with lymph node metastasis (29.1%) than that without lymph node metastasis (7.1%) (P=0.016).
CONCLUSIONNuclear expression of S100A4 is associated with lymph node metastasis of gastric carcinoma.
