Research of the synergetic action of fluid shear stress and 17-beta estradiol on the proliferation of MC3T3-E1 cells.
- Author:
Sixia SHANG
1
;
Linlin YIN
;
Huiqiang SUN
;
Keli JIA
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Animals; Cell Differentiation; Estradiol; Osteoblasts; Rats; Stress, Mechanical
- From: West China Journal of Stomatology 2013;31(1):61-64
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the suitable level and action time of 17-beta estradiol and fluid shear stress (FSS) and their combined effect on the proliferation of rat osteoblasts in vitro.
METHODSMC3T3-E1 osteoblasts were adopted after subcultured and different concentrations of 17-beta estradiol and FSS values were applied respectively on MC3T3-E1, the suitable level of 17-beta estradiol and FSS were selected through MTT and alkaline phosphatase (ALP). Then the two factors at the suitable level were applied simultaneously to MC3T3-E1 to detect the proliferation activity.
RESULTSSeventeen-beta estradiol(10(-8) mol x L(-1) for 5 d and 12 x 10(-5) N FSS for 60 min exhibited better effects on the proliferation activity than the other groups respectively, and the combined effect of both factors was better than any single-factor treated group.
CONCLUSIONBoth 17-beta estradiol and FSS have a suitable threshold in promoting proliferation of osteoblasts, and two-factor treated group exhibits better effect than any other single-factor treated groups. Therefore 17-beta estradiol and FSS have a synergetic action on differentiation and proliferation of osteoblasts.
