Isolation and identification of Streptococcus mutans strains with different genotype from clinical samples.
- Author:
Chenglong WANG
1
;
Donghua SU
;
Jiaojiao LIU
;
Bingfeng CHU
;
Shaohua LI
;
Wei XIA
;
Yanping LUO
;
Jiyong YANG
;
Ding HONGMEI
;
Zhao QIANG
;
Deng BIN
;
Xi QING
;
Xu JUAN
;
Ningsheng S
Author Information
- Publication Type:Journal Article
- MeSH: Dental Caries; Dental Plaque; Genotype; Glucosyltransferases; Humans; Polymerase Chain Reaction; Streptococcus mutans
- From: West China Journal of Stomatology 2013;31(1):80-85
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify Streptococcus mutans (S. mutans) strains from clinical samples.
METHODSPlaque samples from caries-active and caries-free sites on enamel surfaces were obtained and cultivated for S. mutans isolation. Morphology, biochemistry, automatic microorganism analysis system and polymerase chain reaction using primers homologous to surface protein antigen I/II (spaP), glucosyltransferase B (gtfB) and dextranase (dexA) were used to identify S. mutans. Genotype of isolated S. mutans was determined by arbitrarily primed polymerase chain reaction.
RESULTSForty-six strains of S. mutans were obtained from the 32 subjects and were identified as S. mutans by biochemistry, automatic microorganism analysis system and polymerase chain reaction. Five identical genotypes were found by arbitrarily primed polymerase chain reaction.
CONCLUSIONForty-one strains of S. mutans with different genotype were obtained from clinical samples.
