Study on prohibition of high mobility group chromosomal protein N2 against human oral squamous cell carcinoma in vitro.
- Author:
Xiaoqian DONG
1
;
Xiqian LIU
;
Yonghong ZHANG
;
Ping ZHANG
;
Libing LU
;
Xiaoyu LI
;
Ping HUANG
;
Yun FENG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Squamous Cell; Cell Proliferation; High Mobility Group Proteins; Humans; In Vitro Techniques; Mouth Neoplasms; Recombinant Proteins
- From: West China Journal of Stomatology 2013;31(1):91-95
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETake human oral squamous cell carcinoma Tca8113 as experimental model, and study the anti oral squamous cell carcinoma activity of high mobility group chromosomal protein N2 (HMGN2) molecule.
METHODSTrain a large number of recombinant human HMGN2 expression vector Escherichia coli BL21. HMGN2 was expressed under isopropyl-1-thio-beta-galactopyranoside (IPTG) induction and purified by B-PER GST Fusion Protein Purification Kit. A variety of concentrations HMGN2 were added to cell culture medium, cells were tested by MTT, Hoechst 33342 fluorescence staining, flow cytometry assay and Western-blot.
RESULTSMTT results proved that HMGN2 could significantly inhibit human oral squamous cell carcinoma Tca8113 growth. Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis.
CONCLUSIONHMGN2 can promote apoptosis of oral squamous cell carcinoma cells.
