The study of serine/threonine kinase signaling pathway-mediated inhibition of proliferation and invasion of oral squamous cell carcinoma transfected with p53 gene.
- Author:
Jibin GAO
1
;
Xingping LI
;
Jidang JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Squamous Cell; Cell Cycle; Cell Proliferation; Cyclin D1; Genes, p53; Humans; Mitogen-Activated Protein Kinases; Mouth Neoplasms; Protein-Serine-Threonine Kinases; Serine; Signal Transduction; Transfection
- From: West China Journal of Stomatology 2013;31(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the molecular mechanism of proliferation and invasion inhibition in oral squamous cell carcinoma transfected with recombinant adenovirus-p53 (Ad-p53).
METHODSTca8113 cell lines were transfected with Ad-p53. Then the effect of p53 overexpression on cancer cells proliferation and invasion was observed. The expression of mitogen-activated protein kinase (MAPK), serine/threonine kinase (AKT) signaling pathway related proteins, cell cycle and apoptosis related proteins Cyclin D1, P21 and Bcl-2 were detected by Western blotting analysis.
RESULTSAfter transfected with Ad-p53, the proliferation and invasion of Tca8113 cells were significantly inhibited (P < 0.01) and the apoptosis of Tca8113 cells significantly increased (P < 0.001). The results of Western blotting demonstrated that the protein expression of P53 and P21 significantly increased, Cyclin D1 and Bcl-2 protein expression and phosphorylation of AKT protein significantly decreased (P < 0.01).
CONCLUSIONThe AKT signaling pathway may be a key molecular mechanism for proliferation and invasion inhibition of oral squamous cell carcinoma caused by p53. The protein of Cyclin D1, P21 and Bcl-2 may be the downstream targets of AKT signaling pathway. This may provide a new evidence for AKT pathway and downstream targets as a promising therapeutic target for malignant tumors.
