The effects of nicotine on bone calcium and phosphorus content and alkaline phosphatase activity in different part of rat.
- Author:
Haixia ZHAO
1
;
Su MA
;
Li CHEN
;
Peihong LIU
;
Jing XU
;
Lin HOU
;
Rui CHEN
;
Chunlin QIN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Animals; Bone and Bones; Calcium; Male; Nicotine; Phosphorus; Rats; Rats, Wistar
- From: West China Journal of Stomatology 2013;31(3):225-231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the effects of nicotine on the bone calcium and phosphorus content and alkaline phosphatase (ALP) activity in rat alveolar bone and mandible.
METHODSTwenty health male Wistar rats of five weeks of age were randomly assigned to two groups and received daily intraperitoneal injections for three months as follows: Saline solution for control group, nicotine 0.73 mg.kg-l.d-1 for experimental group. The bone calcium phosphorus content were detected by concentrated acid digestion method and the ALP activity was examined by improved Reddi method.
RESULTSCompared to the control group, bone calcium and phosphorus content was lower in the experimental group (P<0.05), ALP activity had no statistical significance(P>0.05). Bone calcium phosphorus and ALP activity in different parts had no statistical significance (P>0.05).
CONCLUSIONThe nicotine reduces calcium phosphorus deposition of jaw bone, but has no obvious influence to ALP activity.
