Engineering the enantioselectivity of biocatalysts.
- Author:
Dunming ZHU
1
;
Qiaqing WU
Author Information
1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. jadezhu@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Biocatalysis;
Epoxide Hydrolases;
genetics;
metabolism;
Esterases;
genetics;
metabolism;
Humans;
Lipase;
genetics;
metabolism;
Protein Engineering;
methods;
Stereoisomerism
- From:
Chinese Journal of Biotechnology
2009;25(12):1770-1778
- CountryChina
- Language:Chinese
-
Abstract:
Wild-type biocatalysts usually show high activity and selectivity towards their native substrates. Since non-native substrates are often used in synthetically useful biocatalytic transformations, it is necessary to engineer enzymes for improved activity, stability and selectivity (chemo-, regio- and stereoselectivity). Herein we give an overview of the recent advances in engineering the enantioselectivity of biocatalysts, with an aim to stimulate further development of this important field in China.
