Effects of mixed carbon sources in cultivation of recombinant Pichia pastoris for polygalacturonate lyase production.
- Author:
Zhihao WANG
1
;
Dongxu ZHANG
;
Jianghua LI
;
Guocheng DU
;
Jian CHEN
Author Information
1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
- Publication Type:Journal Article
- MeSH:
Carbon;
metabolism;
Culture Techniques;
Methanol;
metabolism;
Pichia;
enzymology;
genetics;
growth & development;
Polysaccharide-Lyases;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
Sorbitol;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(12):1955-1961
- CountryChina
- Language:Chinese
-
Abstract:
In order to increase the production and productivity of alkaline polygalacturonate lyase (PGL), we studied the mixed carbon sources feeding strategies during the induction phase by recombinant Pichia pastoris GS 115. Glycerol, sorbitol or lactic acid co-feeding with methanol all enhanced the PGL production. Among all the feeding strategies, the sorbitol co-feeding strategy was most significant. By using this strategy, the PGL activity and productivity reached 1593 U/mL and 16.7 U/(mL-h). Compared to the control, the enhancements of PGL activity and productivity were 84.6% and 45.2% respectively, when we set the sorbitol feeding rate at 3.6 g/(h x L).
