Production of ligninase by co-fermentation of Coprinus comatus and Trichoderma reesei.
- Author:
Chunmei GE
1
;
Juanjuan XU
;
Qinying SUN
;
Jie ZHANG
;
Jingmin CAI
;
Renrui PAN
Author Information
1. Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China.
- Publication Type:Journal Article
- MeSH:
Coprinus;
metabolism;
Culture Techniques;
methods;
Fermentation;
Oxygenases;
biosynthesis;
Plant Stems;
metabolism;
Trichoderma;
metabolism;
Zea mays;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(12):2008-2013
- CountryChina
- Language:Chinese
-
Abstract:
In order to enhance the utilization efficiency, reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration; we studied the ligninase production by Coprinus comatus, Aspergillus niger and Trichoderma reesei through the plate screening. The results showed that C. comatus mixed culture with T. reesei have a good compatibility and higher yields of Laccase. On the basis of this pre-experiment, we studied the optimal conditions of mixed culture for enzyme production. Under the optimal conditions: the inoculation proportion of C. comatus and T. reesei (5:2), the interval of time (12 h), the temperature 260C, the shake rotation speed 150 r/min, fermented for 3 days, the Laccase activity reached 3267.1 U/mL, increased by 106% contrasted with single culture of C. commatus.
