Enhancing ethanol production using thermophilic yeast by response surface methodology.
- Author:
Naikun SHEN
1
;
Qingyan WANG
;
Yan LU
;
Yan QIN
;
Ribo HUANG
Author Information
1. National Engineering Research Center for Non-food Biorefinery, Guangxi Academy ofSciences, Nanning 530007, China.
- Publication Type:Journal Article
- MeSH:
Ethanol;
analysis;
metabolism;
Fermentation;
Glucosidases;
pharmacology;
Hot Temperature;
Manihot;
metabolism;
Substrate Specificity;
Yeasts;
physiology
- From:
Chinese Journal of Biotechnology
2010;26(1):42-47
- CountryChina
- Language:Chinese
-
Abstract:
We optimized the conditions of simultaneous saccharification and fermentation (SSF) from cassava flour into high-concentration ethanol by thermophilic yeast GXASY-10. Based on the single factor experiment, we screened the important parameters by Plackeet-burman design. We used the path of steepest ascent to approach to the biggest region of ethanol production subsequently. Then, we obtained the optimum values of the parameters by Box-Behnken design. The results showed that the important parameters were the liquefaction time, glucosidase dosages and initial concentration of cassava flour (substrate). The optimum technical conditions were as follows: liquefaction time 35 min, glucosidase dosages 1.21 AGU/g substrate and initial substrate concentration 37.62%. Under such optimum conditions, the ethanol yield of 20 L fermentor reached 16.07% (V/W) after 48 h fermentation at 37 degrees C and 100 r/min. The ethanol content increased 33% than that under the original condition.
