Development of a fed-batch process for TNFR-fc producing GS-CHO cells.
- Author:
Li FAN
1
;
Liang ZHAO
;
Yating SUN
;
Tianci KOU
;
Wensong TAN
Author Information
1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
- Publication Type:Journal Article
- MeSH:
Animals;
CHO Cells;
Cell Culture Techniques;
methods;
Cricetinae;
Cricetulus;
Culture Media;
Etanercept;
Glucose;
analysis;
Immunoglobulin G;
biosynthesis;
genetics;
Receptors, Tumor Necrosis Factor;
biosynthesis;
genetics;
Recombinant Fusion Proteins;
biosynthesis;
genetics
- From:
Chinese Journal of Biotechnology
2010;26(2):216-222
- CountryChina
- Language:Chinese
-
Abstract:
TNFR-Fc is an important fusion protein that has great potential in therapeutic and diagnostic applications. We developed an efficient fed-batch process for GS-CHO cells to produce TNFR-Fc. The rationale of this fed-batch process relies on the supply of sufficient nutrients to meet the requirements of cell metabolism. The optimal feed medium was designed through ration design. A metabolically responsive feeding strategy was designed and dynamically adjusted based on the residual glucose concentration determined off-line. In this process, the maximal viable cell density and antibody concentration reached above 9.4x10(6) cells/mL and 207 mg/L, respectively. Compared with the batch process, the newly developed fed-batch process increased the cell yield by 3.4 fold and the final antibody concentration by 3 fold. This fed-batch process would therefore facilitate the production of therapeutic antibody by GS-CHO cells.
