Sequence analysis, expression and antigenicity detection of bovine viral diarrhea virus NS3 gene.
- Author:
Yan LI
1
;
Mingfei NIE
;
Wei WEI
;
Kai WEN
;
Ying JIA
;
Hui HUO
;
Junwei WANG
Author Information
1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Cloning, Molecular;
Diarrhea Viruses, Bovine Viral;
genetics;
immunology;
Escherichia coli;
genetics;
metabolism;
Genetic Vectors;
genetics;
Peptide Hydrolases;
genetics;
immunology;
Phylogeny;
RNA Helicases;
genetics;
immunology;
Recombinant Proteins;
biosynthesis;
genetics;
immunology;
Sequence Analysis, Protein;
Viral Nonstructural Proteins;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2010;26(3):311-316
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we cloned the NS3 gene from bovine viral diarrhea virus (BVDV) VEDEVAC strain. The result showed that the average P-distance of Pestivirus NS3 amino acid sequence was 0.07 and the VEDEVAC strain was classified to BVDV type 1. Using pET-30a(+) as vector and Escherichia coli Rosetta (DE3) as host, we obtained purified recombinant NS3 protein by Ni-NTA affinity chromatography. Western blotting analysis demonstrated that both BVDV positive serum and classical swine fever virus (CSFV) positive serum were able to recognize the recombinant NS3 protein. Indirect-ELISA assay indicated that the protein could be used as detection antigen.
