Expression of porcine interferon-gamma and its safe antiviral assay.
- Author:
Fan HE
1
;
Yuan SUN
;
Jinying GE
;
Miao LI
;
Tianming CHANG
;
Zhigao BU
;
Huaji QIU
Author Information
1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antiviral Agents;
pharmacology;
Baculoviridae;
genetics;
metabolism;
Escherichia coli;
genetics;
metabolism;
Genetic Vectors;
genetics;
Green Fluorescent Proteins;
biosynthesis;
genetics;
Interferon-gamma;
biosynthesis;
genetics;
metabolism;
pharmacology;
Recombinant Proteins;
biosynthesis;
genetics;
pharmacology;
Swine;
Vesiculovirus;
drug effects;
physiology
- From:
Chinese Journal of Biotechnology
2010;26(4):439-447
- CountryChina
- Language:Chinese
-
Abstract:
In order to ensure the biosafety of the IFN-gamma antiviral activity assay, we used a replication-deficient VSV carrying GFP as an interferon sensitive indicator virus (VSVdeltaG*G). The antiviral activities of porcine IFN-gamma expressed in Escherichia coli and in baculovirus on MDBK cells were assessed. The results showed that the antiviral activity of porcine IFN-gamma expressed in baculovirus could reach 10(5) IU/mL, while the porcine IFN-gamma expressed in E. coli showed some antiviral activity (32 IU/mL) after refolding. The results of the VSVdeltaG*G-based antiviral assay were almost identical to that of the VSV*GFP-based assay, suggesting it is highly feasible to use VSVdeltaG*G as a substitute for VSV*GFP, making assays for IFN-gamma antiviral activity safer and more accurate.
