Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells.
- Author:
Wei-dong WU
1
;
Chi-hua FANG
;
Zheng-xin YANG
;
Jia-jin BAO
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; enzymology; metabolism; pathology; Cell Line, Tumor; Female; Humans; Hyaluronoglucosaminidase; genetics; RNA Interference; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Receptor, Epidermal Growth Factor; genetics; metabolism; Transfection
- From: Journal of Southern Medical University 2010;30(6):1260-1263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells.
METHODSReverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR.
RESULTSAfter transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05).
CONCLUSIONTransfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.
