Technical optimization for extracting hypotensive active peptides from Agrocybe aegerita.
- Author:
Shan-gang WU
1
;
Hong-na SUN
;
Jian-hua SUN
;
Dan-kui LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Agrocybe; chemistry; Antihypertensive Agents; isolation & purification; pharmacology; Chromatography, High Pressure Liquid; methods; Dietary Proteins; isolation & purification; pharmacology; Peptides; isolation & purification; pharmacology
- From: Journal of Southern Medical University 2010;30(6):1264-1267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the techniques for extracting hypotensive active peptides from Agrocybe aegerita.
METHODSThe effects of the liquid/solid ratio, extraction time and temperature, pH value of the initial liquid on the extraction percentage (EP) of the hypotensive active peptides were investigated, and inhibition percentage (IP) of the extracts on angiotensin I-converting enzyme was determined.
RESULTSOptimal extraction of the hypotensive active peptides from Agrocybe aegerita was achieved with the liquid/solid ratio of 40:1, extraction time of 3 h, extraction temperature at 30 degrees Celsius;, and pH=8 of the initial liquid. The EP of the hypotensive active peptides from Agrocybe aegerita could reach 87.7% with IP of the extracts on angiotensin I-converting enzyme of 54.0%.
CONCLUSIONThis method is simple and efficient for extracting hypotensive active peptides from Agrocybe aegerita.
