Effect of phosphatase PHLPP1 gene transfer on the proliferation of human umbilical vein endothelial cells.
- Author:
Xing-li WU
1
;
Ding-you YANG
;
Wei YAN
;
Ai-jia SHANG
;
Cai-yi LU
;
Hui-bin XU
;
Shi-wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; Gene Transfer Techniques; Human Umbilical Vein Endothelial Cells; cytology; metabolism; Humans; Nuclear Proteins; genetics; Phosphoprotein Phosphatases; genetics; Transfection
- From: Journal of Southern Medical University 2010;30(6):1298-1300
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the constituent expression of PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1) in human umbilical vein endothelial cells (HUVECs) and the effect of PHLPP1 gene transfer on the proliferation of the cells in vitro.
METHODSCultured HUVECs were transfected with pcDNA3-GFP or pcDNA3HA-PHLPP1 via lipofectamine 2000. The cell proliferation ability was determined by cell counting and MTT colorimetric assay, and Western blotting was used to detect the protein expression of PHLPP1 in the cells.
RESULTSNo PHLPP1 protein was detected in the non-transfected cells or pcDNA3-GFP-transfected cells. pcDNA3HA-PHLPP1 gene transfection significantly increased PHLPP1 expression in the HUVECs (P<0.01), but the cell proliferation status remained unchanged (P>0.05). The absorbance of the cells measured by MTT assay was 0.134-/+0.0152, 0133-/+0.014 and 0.137-/+0.016, with cell counts of (8.293-/+0.962)x10(5), (7.937-/+0.101)x10(5) and (8.127-/+0.112)x10(5), respectively, showing no significant differences between the 3 groups (P>0.05).
CONCLUSIONSPhosphatase PHLPP1 may not be the most important signal protein in the regulation of HUVEC proliferation.
