Effects of sophoridine on the growth and expressions of p53 and vascular endothelial growth factor of transplanted solid tumor SW480 in nude mice.
- Author:
Qi-rui WANG
1
;
Chun-hua LI
;
Xiu-qiong FU
;
Ya-wei LIU
;
Jing TANG
;
Qin FAN
;
Xue-gang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Animals; Cell Line, Tumor; Colonic Neoplasms; metabolism; pathology; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Quinolizines; pharmacology; RNA, Messenger; genetics; Tumor Suppressor Protein p53; metabolism; Vascular Endothelial Growth Factor A; metabolism; Xenograft Model Antitumor Assays
- From: Journal of Southern Medical University 2010;30(7):1593-1596
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of sophoridine on the transplanted solid tumor SW480 and the expression of p53 and vascular endothelial growth factor (VEGF) in the tumor in nude mice.
METHODSThe nude mouse model bearing transplanted solid tumor SW480 was established, and the changes in the volume and weight of the tumor were determined after treatment of the mice with sophoridine. Western blotting and immunohistochemistry were employed to examine the expressions of p53 and VEGF proteins, respectively, and fluorescence quantitative PCR used to detect their mRNA expressions in the tumor tissue.
RESULTSThe volume and weight of the tumor xenograft in sophoridine group decreased in comparison with those in the control group. Sophoridine treatment resulted in lowered expressions of p53 and VEGF at both the protein and mRNA levels in the tumor explants as compared with the control group, with a tumor inhibition rate of 34.07% in nude mice.
CONCLUSIONSophoridine can inhibit the growth of transplanted solid tumor of human SW480 cell line, the mechanism of which involves the inhibition of p53 and VEGF expression.
