Effects of RNA interference against aggrecanase 1 gene on extracellular matrix metabolism of cultured chondrocytes in vitro.
- Author:
Zheng-hui WANG
1
;
Xi-jing HE
;
Zhuang-qun YANG
;
Li WANG
;
Li-xia LI
;
Jun-bo TU
Author Information
- Publication Type:Journal Article
- MeSH: ADAM Proteins; genetics; metabolism; ADAMTS4 Protein; Aggrecans; metabolism; Animals; Cells, Cultured; Chondrocytes; cytology; metabolism; Extracellular Matrix; metabolism; Female; Procollagen N-Endopeptidase; genetics; metabolism; RNA Interference; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Transfection
- From: Journal of Southern Medical University 2009;29(9):1766-1769
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of RNA interference (RNAi)-mediated aggrecanase-1 gene silencing on extracellular matrix metabolism of cultured rat costochondral chondrocytes.
METHODSRat costochondral chondrocyte monolayers were obtained by microdissection and digestion. The growth and morphological changes of the chondrocytes were observed after RNAi of aggrecanase-1 gene. The mRNA expression of aggrecanase-1 was detected by RT-PCR method, and aggrecan content was determined by Western blotting.
RESULTSThe specific inhibition of aggrecanase-1 by RNAi produced no adverse effect on the morphology and growth of the chondrocytes. The mRNA of aggrecanase-1 decreased and aggrecan content increased significantly after transfection of the chondrocytes.
CONCLUSIONInhibition of aggrecanase-1 decreases aggrecan degradation in cultured rat chondrocytes. RNAi technique can be a useful means for studying extracellular matrix metabolism in the cartilage.
