Quantitative real-time PCR for detecting the expression levels of TCR Vgamma subfamilies in patients with graft-versus-host disease following allogenic hematopoietic stem cell transplantation.
- Author:
Xiu-li WU
1
;
Qi-fa LIU
;
Zhi-ping FAN
;
Shao-hua CHEN
;
Xuan DU
;
Jun-jing SUN
;
Yang-qiu LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Case-Control Studies; Female; Graft vs Host Disease; genetics; Hematopoietic Stem Cell Transplantation; adverse effects; Humans; Leukocytes, Mononuclear; metabolism; Male; Middle Aged; Receptors, Antigen, T-Cell, gamma-delta; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; methods; Young Adult
- From: Journal of Southern Medical University 2009;29(9):1790-1792
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of T cell receptor (TCR) Vgamma genes in patients with graft-versus-host disease (GVHD) after allogenic hematopoietic stem cell transplantation (allo-HSCT).
METHODSThe expression levels of the TCR Vgamma I-III genes in peripheral blood mononuclear cells (PBMNCs) from 18 patients with GVHD following allo-HSCT were determined using real-time fluorescence quantitative PCR, with 12 healthy individuals serving as the normal controls.
RESULTSThe expression level of TCR Vgamma II gene in the PBMNCs from patients with GVHD was significant lower than that in the normal controls. The expression patterns of TCR Vgamma I-III subfamilies also underwent alterations in patients with GVHD, and the expression level of TCR Vgamma II gene was significantly lower than that of TCR Vgamma I gene or TCR Vgamma III gene.
CONCLUSIONThe low expression of TCR Vgamma II subfamily might be related to the pathogenesis of GVHD in patients receiving allo-HSCT.
