Expression of oxyntomodulin in bifidobacteria and effect of oxyntomodulin-transformed bifidobacteria on the body weight of obese mice.
- Author:
Ruo-ting LONG
1
;
Wei-sen ZENG
;
Shen-qiu LUO
;
Jiao GUO
;
Yuan-zao LIN
;
Qing-song HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Appetite Depressants; administration & dosage; metabolism; Bifidobacterium; genetics; metabolism; Body Weight; drug effects; Electroporation; Escherichia coli; genetics; metabolism; Mice; Obesity; drug therapy; Oxyntomodulin; administration & dosage; biosynthesis; genetics; Random Allocation; Recombinant Proteins; administration & dosage; biosynthesis; genetics
- From: Journal of Southern Medical University 2009;29(9):1796-1798
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of pBBADs-OXM-transformed bifidobacteria on the body weight of obese mice.
METHODSB. longum was transformed with pBBADs-OXM by electroporation, and arabopyranose-induced oxyntomodulin expression by the bacterium was detected by ELISA. pBBADs-OXM-transformed bifidobacteria was administered orally obese mice on a daily basis with pBBADs-GFP-transformed bifidobacteria as the negative control, and the body weight changes of the mice were observed.
RESULTSOXM was detected by ELISA not only in the supernatant but also the precipitant of the transformed bacterial culture. The body weight of the obese mice fed with pBBADs-OXM-transformed bifidobacteria decreased significantly compared with that of the mice in the obese model group (P<0.05).
CONCLUSIONAdministration of pBBADs-OXM-transformed B.longum can reduce the body weight of obese mice.
