Synthesis of 2-hydroxyl-5-butyramidobenzoic acid and its effect on acetic acid-induced colitis in rats.
- Author:
Rui-cai SHI
1
;
Jian-feng XING
;
Zhao-guo LIU
;
Zhi-zhao YUAN
;
San-qi ZHANG
;
Xiao-li BIAN
;
Ai-guo ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Acetic Acid; Aminobenzoates; chemical synthesis; chemistry; pharmacology; therapeutic use; Animals; Colitis, Ulcerative; chemically induced; drug therapy; Male; Protective Agents; chemical synthesis; pharmacology; therapeutic use; Rats; Rats, Sprague-Dawley; Salicylates
- From: Journal of Southern Medical University 2009;29(9):1843-1845
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the method for synthesis of 2-hydroxyl-5- butyramidobenzoic acid and test its effect on acetic acid-induced colitis in rats.
METHODS2-hydroxyl-5-butyramidobenzoic acid was synthesized from 5-aminosalicylic acid and butyric acid by amidation, esterification and hydrolization. The effect of 2-hydroxyl-5-butyramidobenzoic acid on acetic acid enema-induced colitis in rats was investigated.
RESULTSThe structure of 2-hydroxyl-5-butyramidobenzoic acid was identified by IR and 1H-NMR. After treatment with acetic acid, the colon mucosal damage index (CMDI), fecal occult blood (OB) test, and activity of myelperoxidase (MPO) increased significantly in the rats as compared to the control levels. 2-hydroxyl-5- butyramidobenzoic acid obviously reduced the CMDI and OB, and reduced the level of MPO in the rats with colitis.
CONCLUSIONThe synthesis of 2-hydroxyl-5-butyramidobenzoic acid requires only mild conditions with simple procedures, and the synthesized 2-hydroxyl-5-butyramidobenzoic acid shows obvious therapeutic effects on mucosal damage of in rats with acetic acid-induced colitis.
