Expression of Aurora-B in non-small cell lung cancer and its clinical significance.
- Author:
Wen-rui WANG
1
;
Sheng-sheng YANG
;
Jing-xiang LIN
;
Zhi-yong ZENG
;
Dao-ming LIU
;
Hong-tao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Aurora Kinase B; Aurora Kinases; Carcinoma, Non-Small-Cell Lung; metabolism; pathology; Female; Humans; Lung Neoplasms; metabolism; pathology; Lymphatic Metastasis; Male; Middle Aged; Protein-Serine-Threonine Kinases; genetics; metabolism; RNA, Messenger; genetics; metabolism; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2009;29(9):1853-1856
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of Aurora-B in non-small cell lung cancer (NSCLC) tissues and NSCLC cell lines.
METHODAurora-B expression was examined using immunohistochemical SP method in 91 stage I and 69 stage II-III NSCLC tissues and 40 adjacent tissues. The mRNA and protein expressions of Aurora-B in NSCLC cell lines (A549, H460 and H1299) were examined by RT-PCR and Western blotting, respectively.
RESULTSThe protein expression of Aurora-B was detected in 77.7% (94/121) of the tumor tissues and 9.8% (4/41) of the adjacent tissues, showing a significant difference between them (P<0.01). The positivity rate of Aurora-B protein was not related with the gender and age of NSCLC patients, but with lymph node metastasis, differentiation and histological type of NSCLC (P<0.05). Aurora-B was expressed in all the NSCLC cell lines (A549, H460 and H1299) at both mRNA and protein levels. A549 cells showed the highest expression of Aurora-B.
CONCLUSIONAurora-B protein is highly expressed in NSCLC tissues and cell lines, and may play a crucial role in the invasion, metastasis and development of NSCLC. The mRNA and protein expression levels of Aurora-B differ significantly between different NSCLC cell lines.
