MTT assay for detecting osteosarcoma cell apoptosis.
- Author:
Qiang CHEN
1
;
Jin-hua ZHANG
;
Jian-ting CHEN
;
Zhan-jun SHI
;
Yong-jian DENG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Combined Chemotherapy Protocols; pharmacology; Apoptosis; drug effects; Bone Neoplasms; pathology; surgery; Humans; Osteosarcoma; pathology; surgery; Tetrazolium Salts; Thiazoles; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2009;29(9):1899-1901
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.
METHODSThe osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.
RESULTSMTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.
CONCLUSIONMTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.
