High-performance capillary electrophoresis for determining caffeic acid content in compound Yinhuangjiedu decoction.
- Author:
Zhuo CAI
1
;
Fang YANG
;
Fa YU
;
Lu-jun LOU
Author Information
- Publication Type:Journal Article
- MeSH: Caffeic Acids; analysis; Drugs, Chinese Herbal; chemistry; Electrophoresis, Capillary; methods; Medicine, Chinese Traditional
- From: Journal of Southern Medical University 2009;29(9):1907-1908
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determining the content of caffeic acid in compound Yinhuangjiedu decoction using high-performance capillary electrophoresis (HPCE).
METHODSThe optimized HPCE for determining caffeic acid content utilized a fused-silica capillary tube (75 microm x 60 cm, effective length of 53 cm) with 20 mmol/L borate as the running buffer (pH=9.18), a constant voltage of 12 kV, a sampling time of 5 s at 25 degrees celsius, and UV detection wavelength at 313 nm.
RESULTSThe linear range of caffeic acid was 20-100 microg/ml.
CONCLUSIONHPCE is simple, rapid, and sensitive for quality control of the compound Yinhuangjiedu decoction.
