Effect of soluble total proteins of Zaocys dumnades on interleukin-1beta, tumor necrosis factor-alpha and interleukin-10 expressions in human fibroblast-like synoviocytes in vitro.
- Author:
He-yong WU
1
;
Juan LI
;
Ya-ling LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arthritis, Rheumatoid; Cells, Cultured; Colubridae; Humans; Interleukin-10; secretion; Interleukin-1beta; secretion; Materia Medica; chemistry; pharmacology; Primary Cell Culture; Proteins; isolation & purification; Synovial Membrane; cytology; metabolism; pathology; Tumor Necrosis Factor-alpha; secretion
- From: Journal of Southern Medical University 2009;29(10):1969-1972
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro.
METHODSPrimary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to detect the expressions of IL-1beta, IL-10 and TNF-alpha in the FLS.
RESULTSThe protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01).
CONCLUSIONThe soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS.
