Small interfering RNA-mediated COX-2 gene silencing inhibits the proliferation and migration of human ovarian cancer cell line CAOV-3.
- Author:
Ya-juan SU
1
;
Qian WANG
;
Ling-juan GAO
;
Lei ZHENG
;
Jie BAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Movement; Cell Proliferation; Cyclooxygenase 2; genetics; Female; Humans; Ovarian Neoplasms; genetics; pathology; RNA Interference; RNA, Small Interfering; genetics
- From: Journal of Southern Medical University 2009;29(10):1990-1993
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in the proliferation and migration of human ovarian cancer cells (CAOV-3) after knocking down COX-2 gene by RNA interference.
METHODSThe recombinant plasmid of pGenesil-1-siRNA-COX-2 was constructed and transfected into CAOV-3 cells. The transcription of COX-2 gene was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression of COX-2 was determined by Western blotting . MTT assay was used to investigate the proliferation of the transfected CAOV-3 cells, and the cell migration was evaluated using a transwell migration assay.
RESULTSCOX-2 mRNA and protein levels were significantly reduced after pGenesil-1-siRNA-COX-2 transfection into CAOV-3 cells, which showed obvious reduction in the cell proliferation and migration.
CONCLUSIONRNA interference allows obvious COX-2 gene knocking down in human ovarian cancer cells to result in lowered cell growth rate and migration ability. COX-2 gene may become a new therapeutic target for ovarian cancer.
