Phylogenetic analysis and PCR-restriction fragment length polymophism identification of Salmonella based on grOEL gene sequence.
- Author:
Yu-shan HU
1
;
Jun-hua LIU
;
Xing-lin PANG
;
Shou-yi CHEN
;
Xiao-guang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Typing Techniques; Chaperonin 60; genetics; DNA, Bacterial; genetics; Phylogeny; Polymerase Chain Reaction; methods; Polymorphism, Restriction Fragment Length; Salmonella; classification; genetics; Sequence Analysis, DNA
- From: Journal of Southern Medical University 2009;29(10):2037-2043
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the value of grOEL gene sequence in phylogenetic analysis and typing of Salmonella.
METHODSThe grOEL gene was amplified by PCR, sequenced and analyzed using Bioedit and DNAstar software. The Salmonella strains were identified using PCR-restriction fragment length polymophism (PCR-RFLP).
RESULTSThe conservative and variable regions of grOEL gene of Salmonella serogroup were separately distributed and most of the small mutant regions distributed intermittently among the conservative regions. The phylogenetic tree of Salmonella based on the nucleotides differed from that generated based on the amino acid sequence. O8, O9 and O10 had the closest consanguinity, and 5 patterns were identified by PCR-RFLP.
CONCLUSIONThe grOEL gene can be used as a genetic marker for phylogenetic analysis of Salmonella and also as a target sequence for Salmonella typing identification.
