Small interfering RNA-mediated islet neogenesis associated protein gene silencing inhibits the proliferation of INS-1 islet cells.
- Author:
Jian-ping SHA
1
;
Yao-ming XUE
;
Xuan CHEN
;
Ke LONG
;
Hua-cheng LIANG
;
Dan SANG
;
Rui-rui MAO
;
Zhan LIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Neoplasm; genetics; Biomarkers, Tumor; genetics; Cell Line, Tumor; Cell Proliferation; Insulinoma; pathology; Islets of Langerhans; pathology; Lectins, C-Type; genetics; Pancreatitis-Associated Proteins; RNA Interference; RNA, Small Interfering; genetics; Rats
- From: Journal of Southern Medical University 2009;29(10):2040-2043
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of small interfering RNA (siRNA)-mediated islet neogenesis associated protein (INGAP) gene silencing on the proliferation of islet cells.
METHODSDifferent siRNAs targeting INGAP gene were designed and transfected into INS-1 islet cells, and the expression levels of INGAP mRNA and protein following the transfection were detected using RT-PCR, flow cytometry and Western blotting. The proliferation of the transfected INS-1 cells was evaluated using MTT assay.
RESULTSCompared with those in the irrelevant siRNA, empty vector control, and un-transfected groups, the expression levels of INGAP mRNA and protein in the cells transfected with siRNA6 were reduced significantly. The cell proliferation rate significantly increased after transfection with siRNA6 (P<0.05).
CONCLUSIONsiRNA targeting INGAP can effectively down-regulate INGAP expression and inhibit the proliferation of INS-1 cells.
