Detection of serum autoantibodies to melanocyte and correlation between melanoma antigen recognized by T-cells and vitiligo in children.
- Author:
Jin-ping CHEN
1
;
Hai-pian LI
;
Sheng-hua JIN
;
Jin-tao ZHANG
;
Jun LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Autoantibodies; blood; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Female; Humans; MART-1 Antigen; immunology; Male; Melanocytes; immunology; T-Lymphocytes; immunology; Vitiligo; immunology
- From: Journal of Southern Medical University 2009;29(10):2107-2111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the serum levels of melanocyte antibodies and explore the relation between melanoma antigen recognized by T-cells (MART-1) and vitiligo in children.
METHODSThe serum samples were collected from children with vitiligo to test the autoantibodies, and divided into low- and high-titer group according to the test results. Melanocytes were incubated with the serum samples, and the changes of melanocyte surface antigen were evaluated using specific MART-1 antibody.
RESULTSThe serum melanocyte antibody levels in children with vitiligo were significantly higher than those in normal subjects. The expression level of melanocyte surface antigen MART-1 increased obviously after incubation of the melanocyte with high antibody titer serum samples, and MART-1 was found to specifically bind to specific MART-1 antibody.
CONCLUSIONMelanocytes MART-1 may correlate to the autoimmune mechanism in children with vitiligo.
