Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy.
- Author:
Wei DONG
1
;
Qun-Xing XU
;
Yu-Huan HAN
;
Li-Rong YIN
Author Information
1. Department of Obstetrics, the Second Hospital of Tianjin Medical University, Tianjin, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD;
pharmacology;
Cell Movement;
drug effects;
physiology;
Cells, Cultured;
Endoglin;
Female;
Humans;
Matrix Metalloproteinase 2;
genetics;
metabolism;
Matrix Metalloproteinase 9;
genetics;
metabolism;
Placentation;
physiology;
Pre-Eclampsia;
physiopathology;
Pregnancy;
Pregnancy Trimester, First;
RNA, Messenger;
genetics;
metabolism;
Receptors, Cell Surface;
Trophoblasts;
cytology
- From:
Acta Physiologica Sinica
2011;63(3):267-271
- CountryChina
- Language:Chinese
-
Abstract:
The present study aims to investigate the effects of soluble endoglin (sEng) on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy. Cytotrophoblasts of normal 6 to 8-week pregnancy were cultured by trypsin digestion method, and were incubated with cell culture medium without (control group) and with 10 μg/L sEng (sEng group), respectively for 24 h. The invasive ability was determined by transwell invasion assay, and expressions of MMP-2, MMP-9 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The results showed that the invasive ability of cytotrophoblasts in sEng group was lower than that in control group (P < 0.05). Compared with control group, the expressions of MMP-2 and MMP-9 mRNA and protein of cytotrophoblasts were significantly lower (P < 0.05). In conclusion, sEng may participate in the genesis of preeclampsia by affecting the invasive ability of cytotrophoblasts through regulation of the expression of MMP-2 and MMP-9.
