Genetic cloning and expression of hypoxia inducible factor 1 alpha in high altitude hypoxic adaptation species Tibetan antelope (Pantholops hodgsonii).
- Author:
Fang LIU
1
;
Tana WUREN
;
Lan MA
;
Ying-Zhong YANG
;
Ri-Li GE
Author Information
1. Research Center of High Altitude Medicine, Qinghai University Medical College, Xining 810001, China.
- Publication Type:Journal Article
- MeSH:
Adaptation, Physiological;
genetics;
physiology;
Altitude;
Animals;
Antelopes;
genetics;
physiology;
Cloning, Molecular;
Hypoxia;
physiopathology;
Hypoxia-Inducible Factor 1, alpha Subunit;
genetics;
metabolism;
Lung;
metabolism;
Male;
Myocardium;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Sheep;
Tibet
- From:
Acta Physiologica Sinica
2011;63(6):565-573
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the role of the hypoxia inducible factor 1 alpha (HIF-1α) in the adaptation mechanism to high altitude hypoxia, the cloning of the HIF-1α gene cDNA of Tibetan antelope (Pantholops hodgsonii), using RT-PCR and RACE, was applied, and the comparative analysis of the tissue-specific expressions of HIF-1α among Tibetan antelope, Tibetan sheep and plain sheep was performed using real-time PCR and Western blot. The sequence analysis indicated that the cDNA sequences acquired by cloning from the HIF-1α gene of Tibetan antelope comprised a 2 471-bp open reading frame (ORF) and a 1 911-bp 3'UTR. The similarity between its coding sequence, predicted amino acid sequence and HIF-1α of other mammals exceeded 87%, in which the similarity with cow was up to more than 98%, which showed that this sequence was the cDNA of HIF-1α of Tibetan antelope. The results of real-time PCR and Western blot showed that expressions of HIF-1α mRNA and protein appeared in Tibetan antelope's lung, cardiac muscle and skeletal muscle, with the highest expression in lung. HIF-1α mRNA and protein had obvious differential expression in these tissues. Further research showed that Tibetan antelope and Tibetan sheep possessed higher expressions of HIF-1α protein in the three tissues above-mentioned compared with plain sheep, and the expressions of HIF-1α mRNA and protein in Tibetan antelope's lung, cardiac muscle and skeletal muscle were higher than those of Tibetan sheep. It illustrates that the hypoxic HIF-1α-specific expression is one of the molecular bases of high altitude hypoxia adaptation in Tibetan antelope.
